MTT Assay:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Recombinant:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Lysis:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Chromatin Immunoprecipitation:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Polymerase Chain Reaction:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Purification:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Methylation:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Enzyme-linked Immunosorbent Assay:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
SYBR Green Assay:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
In Vivo:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
Transfection:Article Title: Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation
Article Snippet: .. Lonicerin (C 27 H 30 O 15 , MW: 594.5, >98% purity) was purchased from Chengdu Push Biotech (Chengdu, China); 5-ASA was purchased from Ipsen Pharma (Houdan, France); Myeloperoxidase (MPO) Kit was purchased from Jiancheng Biotech (Nanjing, China); lipopolysaccharide (LPS), phorbol myristate acetate (PMA), muramyldipeptide (MDP), adenosine triphosphate (ATP), nigericin, monosodium urate crystals (MSU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA); DSS was purchased from MP Biomedicals (Solon, OH, USA); recombinant mouse macrophage colony stimulating factor (rmM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA); NLRP3, ASC, IL-1 β , pro-caspase 1, cleaved caspase-1, cleaved IL-1 β , enhancer of zeste homolog 2 (EZH2), LC3B, P62, autophagy-related gene 5 (ATG5) and ATG7 primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); trimethylation of lysine 27 on histone 3 (H3K27me3) antibody was purchased from Abcam (Cambridge, UK, USA); F4/80 antibody was purchased from Wuhan Servicebio Biotech (Wuhan, China); Annexin V-FITC/PI Apoptosis Detection Kit was purchased from ThermoFisher Scientific (Waltham, MA, USA); phosphatase inhibitor cocktail, bafilomycin A1 (BafA1) and chloroquine (CQ) were purchased from Selleck Chemicals (Houston, TX, USA); RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, MD, USA); phenylmethanesulfonyl fluoride (PMSF), RIPA lysis buffer, Chromatin Immunoprecipitation (ChIP) Assay Kit, proteinase K and NP-40 lysis buffer were purchased from Beyotime Biotechnology (Shanghai, China); Genomic DNA Mimi Preparation Kit, QIAquick PCR Purification Kit and the EZ DNA Methylation-Gold Kit were purchased from Tiangen Biotech Co., Ltd. (Beijing, China); mouse and human IL-1 β , IL-18 ELISA Kit were purchased from Dakewe Biotech (Shenzhen, China); Trizol reagent, HiScriptTMQRTSuperMix and AceQTMqPCR SYBR® Green Master Mix were purchased from Vazyme Biotech (Nanjing, China); Enhanced Chemiluminescent Plus Reagent Kit was purchased from Yesen Biotech (Shanghai, China); rhodamine-conjugated goat anti-rat IgG (H + L) secondary antibody, β -actin and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from Bioworld Technology (St Louis Park, MN, USA); the EntransterTM- in vivo transfection regent was purchased from Engreen Biosystem Co., Ltd. (Beijing, China). ..
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